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Recent B2 element insertions in the mouse genome.

A M Roy1, C Gong, D H Kass

  • 1Department of Biochemistry and Molecular Biology, Louisiana State University--Medical Center, New Orleans 70112, USA.

DNA Sequence : the Journal of DNA Sequencing and Mapping
|September 19, 2000
PubMed
Summary
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Researchers discovered a new B2 element insertion in the beta-glucuronidase gene of BalbC mice. This recent genetic event, specific to this mouse strain, offers insights into mobile DNA elements in rodent genomes.

Area of Science:

  • Genomics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • B2 elements are short interspersed repeats amplified in rodent genomes.
  • Previous studies documented mobility in only two B2 elements.
  • Recent B2 element insertions are rare and difficult to identify.

Purpose of the Study:

  • To identify and characterize recent B2 element insertions in mouse genomes.
  • To investigate the insertion dynamics of B2 elements.
  • To understand the evolutionary mechanisms of repetitive DNA in mammals.

Main Methods:

  • Analysis of orthologous loci for a B2 element with high sequence identity to the consensus.
  • Polymerase chain reaction (PCR) amplification using specific flanking primers.

Related Experiment Videos

  • Genomic DNA analysis across various mouse species and laboratory strains.
  • Cloning and sequencing of amplified DNA fragments.

    • Main Results:

    • An additional recent B2 element insertion was identified in intron 4 of the murine beta-glucuronidase gene (Gus-s).
    • The identified B2 element was exclusively present in the Mus musculus BalbC strain.
    • Sequence analysis confirmed the absence of the B2 element in this intron in other mouse strains and revealed sequence variations.
    • Newly inserted B2 elements exhibit low divergence from their consensus sequences.

    Conclusions:

    • The B2 element insertion likely occurred recently within the BalbC mouse population.
    • The findings highlight the potential for recent and localized mobile DNA activity.
    • Recently inserted B2 elements closely resemble their consensus sequences, aiding their detection.