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18F-FDG labelling of human leukocytes.
L A Forstrom1, B P Mullan, J C Hung
1Nuclear Medicine, Department of Diagnostic Radiology, Mayo Clinic, Rochester, MN 55905, USA. forstrom.lee@mayo.edu
Nuclear Medicine Communications
|September 20, 2000
Summary
Researchers explored labeling white blood cells (WBC) with 18F-fluorodeoxyglucose (18F-FDG) for infection imaging. Optimal labeling was achieved using heparin at 37°C with mixing, yielding stable cells for PET imaging applications.
Area of Science:
- Nuclear Medicine
- Cellular Biology
- Medical Imaging
Background:
- Radiolabeled leukocytes are valuable tools for imaging inflammation and infection.
- 18F-fluorodeoxyglucose (18F-FDG) accumulates in metabolically active cells, making it a potential agent for cell labeling.
Purpose of the Study:
- To evaluate the feasibility of labeling leukocytes with 18F-FDG.
- To determine optimal conditions for leukocyte labeling with 18F-FDG for potential use in PET imaging.
Main Methods:
- White blood cells (WBC) were harvested from blood samples.
- Leukocyte labeling with varying concentrations of 18F-FDG was performed under different conditions (anticoagulants, temperature, mixing).
- Labeling efficiency, cell stability, and viability were assessed.
Main Results:
- Satisfactory labeling efficiency (62.7%) was achieved with heparin-saline at 37°C for 30 minutes.
- Mixing during incubation enhanced labeling efficiency to 78.1%.
- 18F-FDG labeled WBCs were stable in plasma for up to 4 hours, with predominant labeling of granulocytes.
Conclusions:
- Leukocyte labeling with 18F-FDG is feasible.
- Optimized labeling conditions enhance efficiency and cell stability.
- This method offers a potential new approach for PET imaging of inflammation and infection.