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Related Experiment Videos

Replication in restenotic atherectomy tissue.

E R O'Brien1, S Urieli-Shoval, M R Garvin

  • 1Vascular Biology Laboratory, Department of Medicine (Cardiology), University of Ottawa Heart Institute, 40 Ruskin Street, Ont., K1Y 4W7, Ottawa, Canada. eobrien@ottawaheart.ca

Atherosclerosis
|September 21, 2000
PubMed
Summary
This summary is machine-generated.

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Cell replication is infrequent in restenotic coronary arteries but more common in peripheral arteries. This study used histone 3 (H3) mRNA in situ hybridization to assess cell proliferation in atherectomy specimens.

Area of Science:

  • Cardiovascular Biology
  • Cellular and Molecular Medicine
  • Vascular Biology

Background:

  • Previous studies indicated infrequent cell replication in restenotic coronary atherectomy specimens.
  • Earlier assessments used proliferating cell nuclear antigen (PCNA) immunocytochemistry, a subjective method, with specimens often resected >3 months post-procedure.
  • A more sensitive method is needed to accurately determine replication rates in these tissues.

Purpose of the Study:

  • To determine the replication profile of human directional atherectomy specimens using a sensitive method.
  • To compare replication rates in coronary versus peripheral artery lesions.
  • To investigate replication in both early and late stages after interventional procedures.

Main Methods:

  • Utilized in situ hybridization for histone 3 (H3) mRNA to detect cell replication.

Related Experiment Videos

  • Analyzed restenotic directional coronary atherectomy specimens from lesions within 3 months of intervention.
  • Assessed larger peripheral artery atherectomy specimens, including both restenotic and primary lesions.

    • Main Results:

    • Replication was detected in 2/17 coronary and 9/12 peripheral artery restenotic lesions.
    • Primary peripheral artery lesions also showed replication (3/11 specimens).
    • Maximum replication percentages were <0.5% (coronary restenotic), <=1.2% (peripheral restenotic), and <0.01% (peripheral primary). Focal areas of high replication were observed.

    Conclusions:

    • Cell replication, assessed by H3 mRNA, is infrequent in restenotic coronary arteries.
    • Peripheral restenotic lesions exhibit more frequent and higher levels of replication, irrespective of the time since the last intervention.
    • While replication is more abundant in restenotic lesions, it is not a dominant factor in the pathophysiology of restenosis.