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Collection of allogeneic peripheral blood stem cells
1Department of Blood and Marrow Transplantation, M.D. Anderson Cancer Center, University of Texas, Houston 77030, USA.
Bailliere'S Best Practice & Research. Clinical Haematology
|September 23, 2000
Summary
Allogeneic stem cell transplantation uses circulating stem cells from healthy donors. This study details the effects of rhG-CSF on stem cell collection and donor safety during apheresis.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Allogeneic stem cell transplantation is vital for treating hematologic malignancies and solid tumors.
- Mobilization of peripheral blood stem cells (PBSCs) from healthy donors is a critical step.
Purpose of the Study:
- To describe the effects of recombinant human granulocyte-colony stimulating factor (rhG-CSF) on CD34+ cell mobilization and subsets.
- To outline safety considerations for cytokine treatment in normal stem cell donors.
- To review stem cell apheresis technology, cell yields, and novel mobilization strategies.
Main Methods:
- Analysis of peripheral blood CD34+ cell and subset concentrations following rhG-CSF administration.
- Documentation of rhG-CSF related side effects in normal donors.
- Review of stem cell apheresis techniques and emerging mobilization agents (Flt3 ligand, anti-VLA4/VCAM-1 antibodies).
Main Results:
- rhG-CSF effectively mobilizes CD34+ cells and specific subsets into peripheral blood.
- Identified rhG-CSF related side effects in normal donors.
- Described apheresis technology and cell yields, alongside novel mobilization approaches.
Conclusions:
- rhG-CSF is effective for stem cell mobilization but requires careful donor safety monitoring.
- Apheresis technology is established, with ongoing research into novel mobilization strategies for improved stem cell collection.