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Recent innovation in capillary electrokinetic chromatography with replaceable charged pseudostationary phases or

B Maichel1, E Kenndler

  • 1Institute of Analytical Chemistry, University of Vienna, Austria.

Electrophoresis
|September 23, 2000
PubMed
Summary
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Charged additives like polymers and dendrimers enhance capillary electrophoresis for neutral analytes. These pseudostationary phases improve separation by forming associates, expanding applications in micellar electrokinetic capillary chromatography (MEKC).

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Capillary Electrophoresis

Background:

  • Capillary electrophoresis typically separates charged analytes.
  • Separating neutral analytes presents a significant challenge in analytical chemistry.
  • Electroosmotic flow (EOF) driven systems offer potential for neutral analyte separation.

Purpose of the Study:

  • To review recent advancements in separating neutral analytes using capillary systems.
  • To explore the use of charged additives as pseudostationary phases.
  • To highlight novel approaches enhancing micellar electrokinetic capillary chromatography (MEKC).

Main Methods:

  • Utilizing charged additives, including soluble polymers (anionic/cationic) and dendrimers.
  • Employing monomeric charged additives to form analyte-additive associates.

Related Experiment Videos

  • Investigating polymers with covalently stabilized structures as permanent micelles.

    • Main Results:

    • Charged additives effectively serve as pseudostationary phases in EOF-driven capillary systems.
    • Polymers and dendrimers demonstrate selective retention and separation of neutral analytes.
    • Permanent micelles offer robustness, increasing MEKC applicability across various mobile phase compositions.

    Conclusions:

    • Charged additives represent a versatile strategy for neutral analyte separation in capillary electrophoresis.
    • Dendrimers show promise for separating lipophilic neutral compounds.
    • Advanced pseudostationary phases significantly broaden the scope and efficiency of MEKC.