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Production and characterization of specific monoclonal antibodies of the human thyroid stimulating hormone

I B Baluja Conde1, A I Brito Moreno, I Amores Sanchéz

  • 1Laboratorio de Anticuerpos Monoclonales, Centro de Immunoensayo, Playa, Ciudad Habana, Cuba. iqmonoclo1@cie.sld.cu

Hybridoma
|September 23, 2000
PubMed

Spleen cells from BALB/c mice immunized with human thyroid stimulating hormone (beta-subunit) were fused with mouse myeloma cells (P3/X63-Ag8) and five hybridomas secreting monoclonal antibodies (MAbs) were obtained. These hybridomas specifically recognize (hTSH) and do not cross-react with the other human glycoprotein hormones such as: luteinizing hormone (LH), follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hcG). The MAbs were of the IgG1 subclass and ascitic fluid from these hybridomas was purified by affinity chromatography on Protein A-sepharose CL-4B column to isolate the IgG1 active fraction. The affinity constant of these MAbs ranged from 3.2 x 10(10) to 1.5 x 10(11) M(-1).

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