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DOPA, dopamine, and DOPAC concentrations in the rat gastrointestinal tract decrease during fasting.

E Eldrup1, E A Richter

  • 1Division of Endocrinology, Herlev Hospital, University of Copenhagen, 2730 Herlev, Denmark. eeldrup@dadlnet.dk

American Journal of Physiology. Endocrinology and Metabolism
|September 23, 2000
PubMed
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Dietary 3,4-dihydroxyphenylalanine (DOPA) and dopamine (DA) significantly impact gastrointestinal and plasma dopamine metabolism. Food sources are crucial for maintaining DOPA and DA levels, influencing overall dopamine sulfate content.

Area of Science:

  • Gastrointestinal Physiology
  • Neuroendocrinology
  • Nutritional Science

Background:

  • The origin of gastrointestinal 3,4-dihydroxyphenylalanine (DOPA) and dopamine (DA) is debated.
  • Investigating the contribution of exogenous food sources versus endogenous synthesis is critical for understanding catecholamine metabolism.

Purpose of the Study:

  • To test the hypothesis that gastrointestinal DOPA and DA are primarily derived from dietary intake.
  • To elucidate the influence of food intake, sympathectomy, and adrenal demedullectomy on tissue catecholamine levels.

Main Methods:

  • Reverse-phase High-Performance Liquid Chromatography (HPLC) with electrochemical detection was used.
  • Tissue concentrations of DOPA, DA, norepinephrine (NE), epinephrine (Epi), and 3,4-dihydroxyphenylacetic acid (DOPAC) were measured.

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  • Experiments involved fed and fasted Wistar rats, as well as sympathectomized and adrenodemedullated rats.

    • Main Results:

    • Sympathectomy and adrenal demedullectomy reduced NE and Epi, but not DOPA levels.
    • The gastrointestinal tract contained substantial amounts of DOPA and DA.
    • Fasting decreased gastric DOPA and DA, and muscle DOPA, but not in other organs.
    • Fasting and sympathectomy differentially affected DOPAC levels in the heart, plasma, and skeletal muscle.

    Conclusions:

    • Dietary DOPA and DA are major determinants of DA metabolism and dopamine-sulfate levels in the gut and plasma.
    • Fasting-induced reduction in muscle DOPA may be linked to decreased insulin stimulation.
    • DOPAC originates from both dietary DA and sympathetic nerve synthesis.