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Comparison of four methods for determining nitrate utilization by cryptococci.

J C Rhodes, G D Roberts

    Journal of Clinical Microbiology
    |January 1, 1975
    PubMed
    Summary
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    A special nitrate broth method accurately identifies cryptococci nitrate utilization in 48 hours. This method is superior to Wickerham broth, Delft plate, and nitrate strip tests for reliable results.

    Area of Science:

    • Microbiology
    • Mycology

    Background:

    • Accurate identification of fungal species is crucial in clinical and research settings.
    • Nitrate assimilation is a key characteristic for the differentiation of yeast species, particularly within the *Cryptococcus* genus.

    Purpose of the Study:

    • To evaluate and compare the efficacy of four different methods for determining nitrate utilization in *Cryptococcus* isolates.
    • To identify the most accurate and efficient method for routine laboratory use.

    Main Methods:

    • The study utilized 236 *Cryptococcus* isolates as test organisms.
    • Four methods were assessed: Wickerham broth, a specialized nitrate broth, Delft plate, and nitrate strip.
    • Results were quantified based on the percentage of correct identifications for nitrate utilization.

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    Main Results:

    • The special nitrate broth method achieved 99% accuracy.
    • Wickerham broth method demonstrated 98% accuracy.
    • Nitrate strip and Delft plate methods yielded 94% and 86% accuracy, respectively.

    Conclusions:

    • The special nitrate broth method is highly accurate and efficient for determining nitrate utilization in *Cryptococcus*.
    • This method provides reliable results within 48 hours, significantly faster than the 14-day Wickerham broth method.
    • The special nitrate broth is recommended as a superior diagnostic tool for yeast identification.