Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Force spectroscopy with single bio-molecules.

H Clausen-Schaumann1, M Seitz, R Krautbauer

  • 1Lehrstuhl für Angewandte Physik and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, D-80799 München, Germany.

Current Opinion in Chemical Biology
|September 28, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-fraction image-guided robotic radiosurgery efficiently controls local prostate cancer recurrence after radical prostatectomy.

BJUI compass·2022
Same author

The Matrilin-3 T298M mutation predisposes for post-traumatic osteoarthritis in a knock-in mouse model.

Osteoarthritis and cartilage·2020
Same author

[Analysis and options for optimization of preoperative assessment for anesthesia at a university hospital].

Der Anaesthesist·2017
Same author

[Polymyalgia rheumatica: What is the current status?].

Zeitschrift fur Rheumatologie·2015
Same author

Dynamic Virus-Bacterium Interactions in a Porcine Precision-Cut Lung Slice Coinfection Model: Swine Influenza Virus Paves the Way for Streptococcus suis Infection in a Two-Step Process.

Infection and immunity·2015
Same author

Occlusal caries extension in relation to visual and radiographic diagnostic criteria: results from a microcomputed tomography study.

Operative dentistry·2015
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

Single-molecule force spectroscopy is a key tool for understanding how force affects biological molecules. Recent advances provide new insights into protein folding, DNA mechanics, and molecular motors.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Biochemistry

Background:

  • Force is a critical parameter influencing the structure and function of numerous biological molecules.
  • Single-molecule force spectroscopy (SMFS) has emerged as a powerful technique for investigating biomolecular behavior at the single-molecule level.
  • Understanding the relationship between molecular structure, function, and applied force is crucial in molecular biology.

Purpose of the Study:

  • To highlight the versatility and recent advancements in single-molecule force spectroscopy.
  • To showcase the application of SMFS in exploring diverse biological systems and processes.
  • To emphasize the importance of force as a functional parameter in biological molecules.

Main Methods:

  • Single-molecule force spectroscopy (SMFS) is employed to probe mechanical properties.

Related Experiment Videos

  • Analysis of binding potentials for receptor-ligand pairs.
  • Investigation of protein folding pathways and dynamics.
  • Characterization of DNA mechanics and the behavior of DNA-binding agents.
  • Study of molecular motor function and force generation.

    • Main Results:

    • Detailed insights into the binding potentials of receptor-ligand pairs have been obtained.
    • Protein folding pathways and intermediate states have been elucidated.
    • The mechanical properties and functioning of molecular motors have been investigated.
    • Significant progress has been made in understanding DNA mechanics.
    • The interactions and effects of DNA-binding agents, including proteins and drugs, have been characterized.

    Conclusions:

    • Single-molecule force spectroscopy is an indispensable tool for dissecting molecular mechanisms.
    • Recent applications have significantly advanced our understanding of biomolecular structure-function relationships.
    • The role of force in biological processes is increasingly recognized and quantifiable through SMFS.