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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Related Experiment Video

Updated: Jun 21, 2026

Universal Molecular Retention with 11-Fold Expansion Microscopy
10:31

Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Yat Ho Chan1, Maddison C Hibbard1, Ruixuan Gao2

  • 1Department of Chemistry, University of Illinois Chicago, Chicago, IL, 60607, USA.

Current Opinion in Chemical Biology
|June 19, 2026
PubMed
Summary

Expansion microscopy (ExM) physically enlarges biological samples to improve imaging resolution. Combining ExM with label-free imaging enables detailed molecular mapping at the subcellular level for advanced spatial biology.

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Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Imaging

Background:

  • Expansion microscopy (ExM) physically expands biological specimens to enhance effective spatial resolution.
  • This technique circumvents the need for hardware modifications in existing imaging systems.
  • Recent advances integrate ExM with label-free imaging modalities.

Purpose of the Study:

  • To review the integration of expansion microscopy with label-free and multimodal imaging techniques.
  • To discuss the advantages and challenges of this combined approach.
  • To provide perspectives on future applications in spatial biology.

Main Methods:

  • Physical expansion of biological specimens.
  • Integration with label-free imaging modalities like stimulated Raman scattering microscopy and mass spectrometry imaging.
  • Multimodal detection of chemical signatures.

Main Results:

  • Achieved enhanced spatial resolution without altering imaging hardware.
  • Enabled single-cell and subcellular molecular mapping.
  • Facilitated label-free and multimodal detection of complex chemical signatures.

Conclusions:

  • The coupling of expansion microscopy with label-free imaging is a powerful emerging approach.
  • This combination offers significant advantages for high-resolution molecular mapping.
  • Further development holds great promise for advancing spatial biology studies.