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[Relationship between rhodamine 6G accumulation and fluconazole resistance in Saccharomyces cerevisiae S288c]
C A Stella1, H I Burgos, R Costanzo
1Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay, Argentina. cstella@fmed.uba.ar
Abstract:
Severe mycotic infections are a source of concern in immunocompromised patients or in those who receive chemotherapy for hematological malignant diseases. One of the causes is referred to the appearance of antimycotic resistant microorganisms. Fluconazole is one of the antimycotic used for invasive mycoses treatment. Therefore it is necessary to evaluate the factors that originate this resistance. In the present report the yeast Saccharomyces cerevisiae S288c was used as a model system. In resistant strains the accumulation of the lipophilic cation Rhodamine 6G, L-leucine uptake and growth inhibition by crystal violet dye were determined. The results presented herein demonstrate the correlation between the membrane potential and the resistance to fluconazole presented by S. cerevisiae strain S288c.
Insights
Antimycotic resistance in Saccharomyces cerevisiae is linked to membrane potential. This study investigated factors contributing to fluconazole resistance in yeast, crucial for treating invasive fungal infections in vulnerable patients.
Area of Science:
- Medical Mycology
- Molecular Biology
- Antimicrobial Resistance
Background:
- Severe fungal infections pose risks to immunocompromised individuals and chemotherapy patients.
- Emergence of antifungal-resistant microorganisms complicates treatment of invasive mycoses.
- Fluconazole is a key antifungal agent, necessitating research into resistance mechanisms.
Purpose of the Study:
- To investigate the factors contributing to fluconazole resistance in Saccharomyces cerevisiae.
- To establish a correlation between membrane potential and antifungal resistance.
- To understand the mechanisms underlying antifungal drug resistance.
Main Methods:
- Utilized Saccharomyces cerevisiae S288c as a model organism.
- Assessed accumulation of Rhodamine 6G, a lipophilic cation.
- Measured L-leucine uptake and growth inhibition by crystal violet dye.
Main Results:
- Demonstrated a correlation between membrane potential and fluconazole resistance in S. cerevisiae.
- Identified specific cellular characteristics associated with resistant strains.
- Provided insights into the biophysical basis of antifungal resistance.
Conclusions:
- Membrane potential is a significant factor in fluconazole resistance in Saccharomyces cerevisiae.
- Understanding these mechanisms can aid in developing strategies against resistant fungal infections.
- The study highlights the importance of membrane potential in antifungal drug efficacy.