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Antibacterial activity evaluation of microcin J25 against diarrheagenic Escherichia coli
S Sable1, A M Pons, S Gendron-Gaillard
1Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences, Université de La Rochelle, La Rochelle, France.
Applied and Environmental Microbiology
|September 30, 2000
Abstract:
The inhibitory activities of known microcins were evaluated against some diarrheagenic Escherichia coli strains. Some antibacterial properties of microcin J25, the most active one, were studied. A rapid two-step purification was performed. The MIC and the minimum bactericidal concentration of J25 against E. coli O157:H7 were 1 and 100 microg ml(-1), respectively. A 10(4)-CFU ml(-1) contamination by this strain was destroyed in milk and meat extract by 6.25 microg of J25 ml(-1) and in half-diluted egg yolk by 50 microg of J25 ml(-1).