Sesquiterpenoids and phenolics from Crepis mollis.

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Organic Chemistry

Background:

• Crepis mollis is a plant species known to produce various secondary metabolites.
• Guaianolides are a class of sesquiterpenes with diverse biological activities.
• Phytochemical investigations are crucial for discovering novel bioactive compounds.

Purpose of the Study:

• To isolate and characterize chemical constituents from the roots of Crepis mollis.
• To identify and determine the structure of a new guaianolide derivative.
• To contribute to the understanding of the phytochemical profile of Crepis mollis.

Main Methods:

• Extraction of secondary metabolites from Crepis mollis roots.
• Chromatographic techniques (e.g., column chromatography) for compound isolation.
• Spectroscopic methods (e.g., NMR, MS) for structural elucidation.

Main Results:

• Isolation of one new guaianolide, 9alpha-hydroxy-4beta, 15, 11beta, 13-tetrahydro-dehydrozaluzanin C.
• Identification of two known guaianolides, eight known guaianolide glycosides, one known germacranolide glycoside, and two known phenylpropanoids.
• Elucidation of the structure and relative configuration of the new compound using spectral data.

Conclusions:

• The roots of Crepis mollis are a rich source of guaianolide-type sesquiterpenes.
• The newly identified compound, 9alpha-hydroxy-4beta, 15, 11beta, 13-tetrahydro-dehydrozaluzanin C, expands the known chemical diversity of this plant.
• Further research may explore the biological activities of these isolated compounds.