Skin grafting across H-Y incompatibility after organ culture

Mouse skin maintained in organ culture for 7 days was transplanted across the H-Y histocompatibility barrier in two inbred strains: C57BL/6 and C3H/An. Cultured grafts were invariably rejected and at a faster rate than noncultured control grafts. Recipients of the cultured male skin grafts rejected subsequent noncultured male skin grafts in accelerated fashion.