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Updated: Aug 1, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
DC-SIGN-ICAM-2 interaction mediates dendritic cell trafficking.
T B Geijtenbeek1, D J Krooshoop, D A Bleijs
1Department of Tumor Immunology, University Medical Center St Radboud, Philips van Leydenlaan 25, Nijmegen 6525 EX, The Netherlands.
Dendritic cells (DCs) use DC-SIGN to attach to ICAM-2 on blood vessels, enabling their crucial migration from blood into tissues to fight infections.
Area of Science:
- Immunology
- Cell Biology
- Vascular Biology
Background:
- Dendritic cells (DCs) are essential immune cells that patrol tissues for foreign antigens.
- Following antigen capture, DCs migrate to lymphoid organs to initiate adaptive immune responses.
- The precise mechanisms governing DC trafficking from blood into tissues are not fully understood.
Purpose of the Study:
- To investigate the role of DC-SIGN in the migration of dendritic cells from the bloodstream into tissues.
- To elucidate the molecular interactions facilitating DC transmigration across the endothelium.
Main Methods:
- Utilized in vitro flow assays to study cell adhesion under shear conditions.
- Investigated the interaction between DC-SIGN and ICAM-2.
- Examined chemokine-induced transmigration of DCs across endothelial monolayers.
Main Results:
- DC-SIGN mediates the tethering and rolling of DC-SIGN-positive cells on the vascular ligand ICAM-2 under shear flow.
- The DC-SIGN-ICAM-2 interaction is critical for regulating DC transmigration across both resting and activated endothelium.
- DC-SIGN is expressed on DC precursors in blood and on immature and mature DCs in various tissues.
Conclusions:
- DC-SIGN plays a central role in the unique trafficking capabilities of dendritic cells.
- This interaction is a key prerequisite for DC emigration from the blood into tissues.
- DC-SIGN is a crucial molecule for immune surveillance and initiation of immune responses.
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