Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection of mannitol formation by bacteria.

Y Chalfan, R Levy, R I Mateles

    Applied Microbiology
    |September 1, 1975
    PubMed
    Summary

    A new test detects mannitol production from fructose by lactic acid bacteria. This method uses hydrochloric acid dehydration and thin-layer chromatography to accurately identify mannitol formation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Reprinted from Biotechnology and Bioengineering, Vol. XI, Issue 3, Pages 269-281 (1968).

    Biotechnology and bioengineering·2000
    Same author

    DOE Privatization.

    Science (New York, N.Y.)·1996
    Same author

    Evaluation of the CELL-DYN 3000 differential.

    American journal of clinical pathology·1992
    Same author

    A study of regional cerebral blood flow and cognitive performance in Alzheimer's disease.

    Journal of neurology, neurosurgery, and psychiatry·1992
    Same author

    Quantification of regional cerebral blood flow (rCBF) using 99Tcm-HMPAO and SPECT: choice of the reference region.

    Nuclear medicine communications·1992
    Same author

    Clinical and neuropathological correlates of depression in Alzheimer's disease.

    Psychological medicine·1992

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Lactic acid bacteria (LAB) are crucial in food fermentation and can produce various metabolites.
    • Mannitol production from fructose is a key metabolic pathway in some LAB strains.
    • Accurate detection of mannitol is important for characterizing LAB and controlling fermentation processes.

    Purpose of the Study:

    • To develop a reliable and straightforward method for detecting mannitol formation from fructose by lactic acid bacteria.
    • To overcome challenges posed by residual fructose interference in mannitol detection.

    Main Methods:

    • A novel detection test was established for identifying mannitol production.
    • Residual fructose interference was mitigated through dehydration using hydrochloric acid.
    • Thin-layer chromatography (TLC) was employed for the separation and visualization of mannitol.

    Main Results:

    • The developed test effectively detects mannitol formation from fructose by lactic acid bacteria.
    • The method demonstrates high specificity by removing interference from residual fructose.
    • Thin-layer chromatography allows for clear identification of the produced mannitol.

    Conclusions:

    • A simple and effective test for detecting mannitol production by lactic acid bacteria has been established.
    • The method's reliance on hydrochloric acid dehydration and TLC ensures accurate results.
    • This technique facilitates the characterization of mannitol-producing LAB strains.

    Related Experiment Videos