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Related Experiment Video

Updated: Jul 23, 2026

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Evaluation of the CELL-DYN 3000 differential.

P J Cornbleet1, D Myrick, S Judkins

  • 1Department of Pathology, Stanford University Medical Center, California 94305.

American Journal of Clinical Pathology
|December 1, 1992
PubMed
Summary

The CELL-DYN 3000 automated differential provides excellent quantitative white blood cell results. However, its suspect flags show limitations in detecting low percentages of abnormal leukocytes and nucleated red blood cells.

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Last Updated: Jul 23, 2026

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Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
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Published on: March 22, 2022

Area of Science:

  • Hematology
  • Clinical Pathology
  • Laboratory Medicine

Background:

  • Automated blood cell differentials are crucial for accurate patient diagnosis.
  • Evaluating the performance of new hematology analyzers is essential for clinical laboratories.
  • The CELL-DYN 3000 is an automated system for complete blood count and differential analysis.

Purpose of the Study:

  • To evaluate the performance of the CELL-DYN 3000 automated differential analyzer.
  • To assess the accuracy of instrument-generated suspect flags for abnormal leukocytes.
  • To determine the reliability of abnormal cell estimates provided by the CELL-DYN 3000.

Main Methods:

  • Comparison of CELL-DYN 3000 results with a 400-cell manual differential.
  • Evaluation of suspect flags for blasts, variant lymphocytes, nucleated red blood cells (NRBCs), and immature granulocytes (IGs).
  • Analysis of sensitivity and specificity for detecting various abnormal leukocyte populations and platelet clumping.

Main Results:

  • Excellent quantitative white blood cell differential results were observed.
  • Combined specificity for BLAST, VARIANT LYMPH, NRBC, WBC, or DIFF flags was 82.6%.
  • Combined sensitivity for detecting abnormal leukocytes or platelet clumping was 81.6%.
  • The instrument struggled to flag low percentages (< or = 5%) of nucleated erythrocytes, lymphoid blasts, hairy cells, or >5% reactive lymphocytes.
  • Specificity of the immature granulocyte (IG) flag was 94.9% for IG counts >= 3%.
  • Sensitivity of the IG flag varied from 41.7% (>= 1% IG) to 100% (>= 3% IG).
  • Flagging performance for band neutrophils showed poor sensitivity and specificity.

Conclusions:

  • The CELL-DYN 3000 offers reliable quantitative differential analysis.
  • Instrument flags require careful review, especially for low-level abnormalities.
  • Further optimization may be needed for flagging specific abnormal cell types and band neutrophils.