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New HLA-DRB1* nucleotide sequence of Italian origin: HLA-DRB1*13022
M Martinetti1, C Pizzochero, M Asti
1Servizio di Immunoematologia e Trasfusione e Centro d'Immunologia dei Trapianti, IRCCS Policlinico S. Matteo, Pavla, Italy. m.martinetti@smatteo.pv.it
Abstract:
High-resolution polymerase chain reaction using sequence-specific primer typing of the HLA-DRB1 gene of an Italian patient waiting for unrelated bone marrow transplantation revealed a new allelic variant of HLA-DRB1*13. Sequencing the exon 2 of DRB1* gene demonstrated a G-->C transition at the nucleotide 216 resulting in a silent mutation at codon 72: CGG-->CGC. The closest sequence was the HLA-DRB1*1302 and the new allele was named HLA-DRB1*13022. This variant was carried by the haplotype HLA-A*24; Cw*0702; B*39; DRB1*13022; DRB3*0301; DQA1*0102; DQB1*0604 as demonstrated by a family study.