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A new positive/negative selectable marker, puDeltatk, for use in embryonic stem cells.

Y T Chen¹, A Bradley

¹Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Genesis (New York, N.Y. : 2000)

|October 6, 2000

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A new selection tool, puDeltatk, was created for genetically modifying cells. This tool allows researchers to easily select and identify modified cells, aiding in various stem cell research applications.

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Developing efficient selectable markers is crucial for genetic engineering.
Existing markers often have limitations in germline transmission or application scope.

Purpose of the Study:

To create and characterize a novel bifunctional positive/negative selection cassette, puDeltatk.
To evaluate its utility in murine embryonic stem cells and germline transmission.

Main Methods:

Generation of the puDeltatk fusion protein.
Transfection of murine embryonic stem cells.
Assessment of drug resistance (puromycin) and sensitivity (FIAU).
Analysis of germline transmission in male mice.

Related Experiment Videos

Main Results:

The puDeltatk cassette confers puromycin resistance and FIAU sensitivity.
puDeltatk is effectively transmitted through the male germline.
Demonstrated utility in murine embryonic stem cell applications.

Conclusions:

puDeltatk serves as a versatile and convenient positive/negative selectable marker.
Its efficient germline transmission broadens its applicability in genetic studies.