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The rabies virion-associated 100-kDa polypeptide (VAP100) is a host-derived minor component of the viral envelope

S Xiao1, K Komiya, T S Tochikura

  • 1Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.

Microbiology and Immunology
|October 6, 2000
PubMed

Insights

We identified a 100-kDa polypeptide (VAP100) in rabies virions using a specific antibody. VAP100 is a cell membrane-associated protein involved in rabies virus assembly and incorporation into virions.

Area of Science:

  • Virology
  • Cell Biology
  • Immunology

Background:

  • Rabies virus is a significant human pathogen.
  • The composition of rabies virions is not fully understood.
  • A 100-kDa polypeptide (VAP100) was previously detected in rabies virions.

Purpose of the Study:

  • To characterize the 100-kDa polypeptide (VAP100) found in rabies virions.
  • To investigate the cellular localization and function of VAP100.
  • To determine the association of VAP100 with rabies virus infection.

Main Methods:

  • Monoclonal antibody (mAb) #16743 production and characterization.
  • Immunoblot analysis (standard and luminescent).
  • Immunoprecipitation of radiolabeled proteins.
  • Fluorescent antibody (FA) staining and double staining.

Main Results:

  • mAb #16743 specifically recognized the VAP100 antigen.
  • VAP100 was detected in rabies virions and cell lysates using sensitive methods.
  • VAP100 is a membrane-associated cellular component.
  • Rabies virus infection altered VAP100 distribution, showing colocalization with viral envelope antigens.
  • VAP100 is likely involved in the efficient incorporation of VAP100 into the virion.

Conclusions:

  • VAP100 is a cellular component intimately associated with rabies virus envelope proteins.
  • VAP100 plays a role in the assembly and/or incorporation of rabies virions.

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