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Related Experiment Videos

The Vibrio cholerae O1 chromosomal integron.

Christopher A Clark1, Leanne Purins1, Pranom Kaewrakon1

  • 1Microbial Pathogenesis Unit, Department of Microbiology and Immunology, University of Adelaide, Adelaide, South Australia 50051.

Microbiology (Reading, England)
|October 6, 2000
PubMed
Summary

Researchers discovered a new class of integrons in Vibrio cholerae, featuring exceptionally large arrays of Vibrio cholerae repeats (VCRs). This finding expands our understanding of gene capture and recombination systems in bacteria.

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Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Integrons are genetic elements known for capturing and expressing genes, primarily associated with antibiotic resistance.
  • Previously, integron arrays typically contained fewer than five gene cassettes.
  • The Vibrio cholerae repeat (VCR) was a newly discovered element in Vibrio cholerae.

Purpose of the Study:

  • To investigate the structure and organization of large gene cassette arrays in Vibrio cholerae.
  • To identify and characterize novel integron classes and associated integrases within Vibrio cholerae.
  • To explore the potential for site-specific recombination mediated by integrons in Vibrio cholerae.

Main Methods:

  • Developed a method for cloning the ends of large cassette arrays.

Related Experiment Videos

  • Utilized Southern hybridization and restriction mapping to physically map VCR arrays.
  • Performed sequence determination to identify integrase genes and characterize integron structures.
  • Conducted recombination assays to demonstrate integrase-mediated activity.
  • Analyzed genomic DNA from various Vibrio species using Southern analysis.

    • Main Results:

    • Identified a novel class 4 integron containing the intI4 integrase gene in Vibrio cholerae.
    • Characterized extensive VCR arrays, estimated to contain approximately 150 gene cassettes within a 120 kb genomic region.
    • Demonstrated IntI1-mediated recombination between VCRs.
    • Identified evidence for a novel class 5 integron in Vibrio mimicus.
    • Observed significant restriction fragment length polymorphism in VCR hybridization patterns across different Vibrio strains.

    Conclusions:

    • Vibrio cholerae harbors exceptionally large integron arrays, significantly larger than previously described.
    • The discovery of class 4 and class 5 integrons expands the known diversity of these gene capture systems.
    • Integron-like site-specific recombination occurs within Vibrio cholerae, with implications for genome evolution and adaptation.
    • VCRs and associated integrons are widespread but show considerable genomic variation among Vibrio species.