Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 22, 2026

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Published on: July 13, 2014

Low levels of ethanol stimulate and high levels decrease phosphorylation in microtubule-associated proteins in rat

B Ahluwalia¹, S Ahmad, O Adeyiga

¹Laboratory of Endocrine Research, Department of Obstetrics and Gynecology, College of Medicine, Howard University, Washington DC 20060, USA.

Alcohol and Alcoholism (Oxford, Oxfordshire)

|October 7, 2000

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Expression sites and developmental regulation of genes encoding (1→3,1→4)-β-glucanases in germinated barley.

Planta·2013

Same author

Regional delivery of radioaerosol.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2013

Same author

Screening for high- and moderate-risk drinking during pregnancy: a comparison of several TWEAK-based screeners.

Alcoholism, clinical and experimental research·2001

Same author

Alcohol modulates cytokine secretion and synthesis in human fetus: an in vivo and in vitro study.

Alcohol (Fayetteville, N.Y.)·2000

Same author

CrkL and CrkII participate in the generation of the growth inhibitory effects of interferons on primary hematopoietic progenitors.

Experimental hematology·1999

Same author

Role of AKT1 in 17beta-estradiol- and insulin-like growth factor I (IGF-I)-dependent proliferation and prevention of apoptosis in MCF-7 breast carcinoma cells.

Biochemical pharmacology·1999

Ethanol affects microtubule-associated proteins (MAPs) phosphorylation, primarily stimulating MAP2. This study investigated ethanol

Area of Science:

Neuroscience
Cell Biology
Biochemistry

Background:

Microtubule-associated proteins (MAPs) regulate microtubule (MT) function through phosphorylation.
Ethanol's impact on neuronal cytoskeleton phosphorylation is not fully understood.

Purpose of the Study:

To investigate the hypothesis that ethanol affects MAPs phosphorylation.
To determine the dose-dependent effects of ethanol on MAPs phosphorylation.

Main Methods:

Prepared microtubules (MT) from rat brain.
Assessed kinase activity using [gamma(32)P]ATP with varying ethanol concentrations.
Investigated the role of cAMP-dependent protein kinase.

Main Results:

Ethanol predominantly stimulated MAP2 phosphorylation in a dose-dependent manner.

More Related Videos

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Published on: February 11, 2020

Flypub To Study Ethanol Induced Behavioral Disinhibition and Sensitization

Flypub To Study Ethanol Induced Behavioral Disinhibition and Sensitization

Published on: May 18, 2020

Related Experiment Videos

Last Updated: Jul 22, 2026

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Published on: July 13, 2014

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Published on: February 11, 2020

Flypub To Study Ethanol Induced Behavioral Disinhibition and Sensitization

Flypub To Study Ethanol Induced Behavioral Disinhibition and Sensitization

Published on: May 18, 2020

Low ethanol levels increased kinase activity, while high levels decreased it.

MAP1 phosphorylation was less affected; tubulin and neurofilament showed no biphasic response.

Conclusions:

Ethanol significantly stimulates MAP2 phosphorylation.
The observed effects are dose-dependent and not mediated by cAMP-dependent protein kinase.