Related Experiment Video

Updated: Oct 11, 2026

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
07:13

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Published on: March 26, 2014

Adenovirus-mediated p16INK4 gene transfer significantly suppresses human breast cancer growth

I Campbell1, A Magliocco, T Moyana

  • 1Department of Microbiology, Saskatoon Cancer Center, University of Saskatchewan, Saskatoon, Canada.

Cancer Gene Therapy
|October 7, 2000
PubMed

Insights

The p16INK4 tumor suppressor gene effectively inhibits breast cancer cell growth by blocking cell cycle progression and inducing apoptosis. This study highlights its potential for breast cancer gene therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • The p16INK4 tumor suppressor gene inhibits cyclin-dependent kinase 4.
  • Homologous deletion of p16INK4 is frequent in human breast cancer.
  • Previous studies show p16INK4 gene transfer inhibits various human tumors.

Purpose of the Study:

  • To investigate the tumor-suppressive function of p16INK4 in human breast cancer.
  • To evaluate the efficacy of p16INK4 gene transfer for breast cancer therapy.
  • To examine the role of p16INK4 in breast cancer cell cycle regulation and apoptosis.

Main Methods:

  • Adenovirus-mediated gene delivery system used to introduce wild-type p16INK4 gene.
  • Expression of p16INK4 in breast cancer cell lines lacking endogenous p16INK4 protein.
  • In vitro and in vivo assays to assess tumor cell proliferation, cell cycle, and apoptosis.

Main Results:

  • Introduced p16INK4 expression blocked tumor cell entry into S phase of the cell cycle.
  • p16INK4 gene transfer induced tumor cell apoptosis.
  • Significant inhibition of tumor cell proliferation observed both in vitro and in vivo.

Conclusions:

  • p16INK4 functions as a potent tumor suppressor gene in breast cancer.
  • p16INK4 gene therapy demonstrates significant potential for treating human breast cancer.
  • Further research into p16INK4-based therapeutic strategies for breast cancer is warranted.

More Related Videos

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland06:29

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

Published on: June 7, 2019

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection05:36

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Published on: February 10, 2023

Related Concept Videos

Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...