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Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
Monocytes are target cells for IL-10 induction by HIV-1 Nef protein
N Tangsinmankong1, N K Day, R A Good
1Department of Pediatrics, University of South Florida College of Medicine, All Children's Hospital, St. Petersburg, FL 33701, USA.
Cytokine
|October 12, 2000
Summary
Human Immunodeficiency Virus (HIV) Nef protein specifically induces Interleukin-10 (IL-10) production in monocytes, not T or B cells. This finding clarifies cell-specific immune responses to HIV components.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- Interleukin-10 (IL-10) is implicated in various diseases and elevated in Human Immunodeficiency Virus (HIV)-infected individuals.
- Previous research indicated HIV Nef protein induces IL-10 mRNA and production in cell lines and peripheral blood mononuclear cells (PBMCs).
- Calmodulin antagonist W-7 was shown to inhibit Nef-induced IL-10.
Purpose of the Study:
- To investigate the specific cell types within PBMCs responsible for IL-10 production induced by HIV Nef.
- To compare the effects of HIV Nef and Staphylococcal Enterotoxin A (SEA) on IL-10 expression in isolated human immune cells.
Main Methods:
- Isolation of T lymphocytes, B lymphocytes, and monocytes from PBMCs of healthy donors.
- Treatment of isolated cell populations with HIV Nef or SEA for 3-24 hours.
- Analysis of IL-10 production and mRNA gene expression in treated cells.
Main Results:
- HIV Nef significantly induced IL-10 production and mRNA expression in monocytes.
- HIV Nef did not induce IL-10 production or mRNA expression in T or B lymphocytes.
- Staphylococcal Enterotoxin A (SEA) induced IL-10 production and mRNA expression in T lymphocytes but not in monocytes or B lymphocytes.
Conclusions:
- Monocytes are the primary responders to HIV Nef-induced IL-10 production and gene expression.
- T lymphocytes are the primary responders to SEA-induced IL-10 production and gene expression.
- These findings highlight cell-specific immune responses to viral proteins and microbial toxins.
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