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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

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Published on: September 17, 2012

Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E

J C de Nooij1, K H Graber, I K Hariharan

  • 1Massachusetts General Hospital Cancer Center, Building 149, Charlestown, MA 02129, USA.

Mechanisms of Development
|October 12, 2000
PubMed

Insights

Cyclin E accumulation drives the expression of Dacapo, a cell cycle inhibitor, in Drosophila. This discovery reveals a key mechanism controlling cell division arrest before differentiation.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • The Cip/Kip family of cyclin-dependent kinase inhibitors (CKIs) are crucial for cell cycle arrest preceding terminal differentiation.
  • The precise mechanisms activating CKI expression during this process remain largely unknown.

Purpose of the Study:

  • To investigate the regulatory mechanism of dacapo, a Drosophila Cip/Kip CKI, during cell cycle arrest.
  • To elucidate the relationship between Cyclin E and Dacapo expression.

Main Methods:

  • Analysis of dacapo RNA and protein accumulation in Drosophila.
  • Investigating the effect of Cyclin E overexpression on dacapo expression.
  • Studying the temporal coupling of Cyclin E and Dacapo during ovarian endocycles.

Main Results:

  • Dacapo RNA and protein accumulation is dependent on Cyclin E.
  • Increased Cyclin E expression can induce dacapo expression.
  • Cyclin E and Dacapo protein levels oscillate in tight coordination during ovarian endocycles.

Conclusions:

  • Cyclin E/Cdk activity plays a critical role in inducing Dacapo expression.
  • Dacapo expression is regulated within specific temporal windows permissive for its induction by Cyclin E/Cdk activity.

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