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Updated: Aug 12, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
Published on: April 29, 2011
E2F transcription factors are differentially expressed in murine gametes and early embryos
A Palena1, R Mangiacasale, A R Magnano
1Centro di Genetica Evoluzionistica CNR, c/o Dipartimento di Genetica e Biologia Molecolare, Università "La Sapienza", Via degli Apuli 4, 00185, Roma, Italy.
Abstract:
We have examined the murine genes encoding transcription factors E2F1, -3, -5 and -6 in gametes and early embryos. All genes are expressed as maternal transcripts and all are efficiently transcribed after the blastocyst stage. Between those two stages, each E2F mRNA is transcribed with a distinctive and unique pattern. E2F proteins are also differentially expressed and compartmentalized in pre-implantation embryos.
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