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Related Experiment Videos

Reproducibility of the analytab (API 20E) system.

D A Butler, C M Lobregat, T L Gavan

    Journal of Clinical Microbiology
    |October 1, 1975
    PubMed
    Summary
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    The Analytab (API 20E) system shows high reproducibility for identifying Enterobacteriaceae at the genus-species level. However, individual biochemical test results varied, impacting overall strain identification accuracy.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Bacterial Identification

    Background:

    • The Analytab (API 20E) system is widely used for bacterial identification.
    • Ensuring the reproducibility of diagnostic tests is crucial for accurate patient care.

    Purpose of the Study:

    • To evaluate the reproducibility of the API 20E system for Enterobacteriaceae identification.
    • To identify factors affecting the variability of API 20E test results.

    Main Methods:

    • 110 clinical isolates of Enterobacteriaceae were tested.
    • Each isolate was identified twice by two technologists.
    • Reproducibility of genus-species identification and individual biochemical tests was assessed.
    • Variable strains were re-tested under modified incubation times, inoculum concentrations, and technologist interpretations.

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    Main Results:

    • Genus-species identification reproducibility was 97.3%.
    • Identical reactions across all 20 biochemical tests were observed in only 55.5% of strains.
    • Individual biochemical test reproducibility ranged from 89% to 100%.
    • Optimized conditions (22h incubation, 10^7 CFU/ml inoculum) reduced technologist interpretation variation.

    Conclusions:

    • While the API 20E system is reliable for genus-species identification, variability in biochemical tests necessitates careful interpretation.
    • Standardizing inoculum size, incubation time, and technologist interpretation can minimize errors.
    • Understanding test reproducibility aids in confirming strain identity when API profiles differ slightly.