Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput single-molecule spectroscopy in free solution.

Y Ma1, M R Shortreed, E S Yeung

  • 1USDOE and Department of Chemistry, Iowa State University, Ames 50011, USA.

Analytical Chemistry
|October 12, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selectivity in the laser-induced photochemistry of iodine + acetylene in the gas phase.

Journal of the American Chemical Society·2012
Same author

An absorption detection approach for multiplexed capillary electrophoresis using a linear photodiode array.

Analytical chemistry·2011
Same author

People: therese m. Cotton (1939-1998).

Analytical chemistry·2011
Same author

Laser Vaporization/Ionization Interface for Capillary Electrophoresis-Time-of-Flight Mass Spectrometry.

Analytical chemistry·2011
Same author

Characterization of Nonbonded Poly(ethylene oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow.

Analytical chemistry·2011
Same author

Two-dimensional spatial imaging based on acoustooptical deflectors.

Applied optics·2010

This study introduces a rapid single-molecule imaging technique that identifies molecules in solution by their unique fluorescence spectra. This method allows for high-throughput analysis of individual molecules without immobilization, enabling disease marker screening and molecular interaction monitoring.

Area of Science:

  • Spectroscopy
  • Biophysics
  • Molecular Imaging

Background:

  • Traditional single-molecule analysis often requires immobilization or spatial confinement.
  • Previous spectral analysis relied on limited wavelength selection, hindering detailed identification.

Purpose of the Study:

  • To develop a high-speed, high-throughput single-molecule imaging technique for identifying molecules in free solution.
  • To leverage full fluorescence emission spectra for enhanced molecular discrimination.

Main Methods:

  • Development of a single-molecule imaging system capable of recording entire fluorescence emission spectra.
  • Utilizing millisecond data acquisition for analysis of molecules in free solution.
  • Demonstration with lambdaDNA and biotinylated DNA-avidin-R-phycoerythrin interactions.

Related Experiment Videos

Main Results:

  • Successful differentiation of individual lambdaDNA molecules labeled with different dyes (YOYO-I, POPO-III).
  • Simultaneous imaging and identification of distinct reactant and product molecules in a DNA-protein interaction.
  • Demonstrated capability to distinguish molecules based on their unique spectroscopic signatures.

Conclusions:

  • The developed technique enables high-throughput, real-time identification of single molecules in solution.
  • This method offers a powerful tool for screening disease markers and monitoring molecular interactions.
  • The ability to analyze full spectra without immobilization significantly advances single-molecule analysis capabilities.