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Solid-phase synthesis of bleomycin group antibiotics. Elaboration of deglycobleomycin A(5)
C J Leitheiser1, M J Rishel, X Wu
1Departments of Chemistry and Biology, University of Virginia, Charlottesville, Virginia 22901, USA.
Organic Letters
|October 13, 2000
Abstract:
The solid-phase syntheses of two deglycobleomycin A(5) analogues were achieved using a commercially available polystyrene resin containing triphenylmethyl-linked spermidine. The final products were deblocked and released from the resin, analyzed, and purified by C(18) reversed phase HPLC and characterized by high-field (1)H NMR spectroscopy and mass spectrometry. The purified products relaxed supercoiled plasmid DNA in a concentration-dependent fashion and to the same extent as authentic material derived from natural BLM A(5).