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Plastic foil technique attenuates inflammation in mesenteric intravital microscopy
G Horstick1, T Kempf, M Lauterbach
12nd Medical Clinic, Institute for Neurosurgical Pathophysiology, Langenbeckstrasse 1, Mainz, 55131, Germany. horstick@mail.uni-mainz.de
The Journal of Surgical Research
|October 20, 2000
Summary
The plastic foil technique is superior for prolonged rat mesenteric intravital microscopy, maintaining stable conditions unlike the organ bath method which causes significant inflammatory responses and data distortion.
Area of Science:
- Physiology
- Microcirculation Research
- Surgical Techniques
Background:
- Surgical preparation for intravital microscopy can induce an inflammatory response, complicating data interpretation.
- Prolonged observation of mesenteric microcirculation requires optimized experimental conditions.
Purpose of the Study:
- To evaluate different experimental techniques for extended intravital microscopy of rat mesenteric microcirculation.
- To identify methods that minimize surgical trauma and inflammatory responses.
Main Methods:
- Exteriorization of the rat mesentery via laparotomy.
- Comparison of organ bath versus modified plastic foil techniques.
- Analysis of hemodynamic, metabolic, respiratory, and microcirculatory parameters.
Main Results:
- The plastic foil technique maintained stable baseline values for 5 hours.
- The organ bath technique showed decreased venular velocity, wall shear rates, and abdominal blood flow.
- Leukocyte adhesion increased, and metabolic parameters (base excess, pCO2) were significantly altered in the organ bath technique.
Conclusions:
- The plastic foil technique is superior for mesenteric intravital microscopy.
- This technique ensures stable metabolic, hemodynamic, and microcirculatory conditions.
- It minimizes the inflammatory response associated with surgical preparation.