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Updated: Sep 17, 2026

High-throughput Gene Tagging in Trypanosoma brucei
Published on: August 12, 2016
Trypanosoma brucei: four tandemly linked genes for fatty acyl-CoA synthetases
D W Jiang1, R Ingersoll, P J Myler
1Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA.
Abstract:
We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the "ACS signature motif." Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes.
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