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[Distribution of K5, K10 in transgenic mouse with lac Z reporter gene]
1Department of Dermatology, Second Affiliated Hospital, Xian Medical University.
Objective:
To detect the distribution of K5, K10 in live mouse.
Methods:
lacZ gene, which encodes beta-galactosidase, as a reporter gene, bovine K5, K10 as promoter, containing Sal I fragment, was inserted into the Sal I sites of PLGZ and PLBS-2, respectively. The constructs were microinjected into the mice fertilized eggs, and then PCR positive ones were kept. The next generations could be used for experiments. Frozen sections of transgenic mice skin, tail were stained with 0.1% X-gal solution at 37 degrees C overnight and counterstained with nuclear fast red.
Result:
In sguamous epidermis, K5 was distributed in basal and outer root sheath, K10 in suprabasal and inner root sheath. K5 and K10 were also distributed in some other organs except heart. No K5 was seen in the brain.
Conclusion:
lacZ transgenic animal is helpful in the study of gene mutation and treatment. The recognition of K5 and K10 distribution could be a primary step to probe the mechanism of some related diseases and cancers.