Related Experiment Videos
[Vascular endothelial growth gene therapy for narrowing of rabbit iliac artery]
1Department of Biochemistry and Molecular Biology, Beijing Medical University.
Objective:
To study the mechanism of renarrowing and vascular endothelial growth factor (VEGF) gene therapy to prevent the restenosis caused by balloon angioplasty.
Methods:
According to the special function of VEGF that promepts the proliferation of endothelial cell (EC), we constituted an eukaryotic expression vector of pcDNA3/VEGF, and added the pcDNA3/VEGF to the surface of polylysine-balloon. We examined the results of the test by in situ blot, eletromagnetic blood flow mechine, scanning eletromicroscope, and light microscope.
Results:
There was higher expression of VEGF mRNA and iliac artery intima in the VEGF gene group. The extent of endothelialization was larger, the ratio of intima to media (I/M) was smaller, and the blood flow was more sufficient than in the vector control group.
Conclusion:
VEGF gene local delivery could stimulate the proliferation of EC, accelerate the endothelialization of injury-artery, and inhibite the proliferation of smooth muscle cell (SMC).