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Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
[Comparative study of the physico-chemical properties of chitosans with varying degree of polymerization in neutral
V N Davydova1, I M Ermak, V I Gorbach
1Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, Russia.
Abstract:
The physicochemical properties of chitosan samples with high (130 kD) and low (30 kD) molecular masses in neutral aqueous solutions (pH 6.0) were studied by the methods of high-speed and equilibrium sedimentation, viscosimetry, and NMR and UV spectroscopies. Differences in the hydrodynamic characteristics of the samples were revealed. It was found that low-molecular-weight chitosan represents flexible linear macromolecules which undergo conformational changes upon temperature increase. The high-molecular-weight chitosan forms more rigid asymmetric structures whose conformation does not vary significantly with temperature increase. It was found that the high-molecular-weight chitosan has a higher constant of binding to the anionic dye tropeoline 000-II, which can be explained by different conformations of their macromolecules in solution.
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