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Envelope proteins in Neisseria.

R R Russell, K G Johnson, I J McDonald

    Canadian Journal of Microbiology
    |October 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    This study analyzed Neisseria sicca cell envelope proteins using SDS-PAGE, revealing distinct outer membrane protein profiles. These profiles aid in classifying non-pathogenic Neisseria species.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacterial Cell Envelope Research

    Background:

    • The cell envelope is crucial for bacterial structure and function.
    • Neisseria species possess complex cell envelopes with outer membranes containing various proteins.
    • Understanding these proteins is key to bacterial classification and potential therapeutic targets.

    Purpose of the Study:

    • To characterize the cell envelope proteins of Neisseria sicca ATCC 9913.
    • To investigate the relationship between cell envelope protein composition and outer membrane properties.
    • To evaluate the utility of cell envelope protein profiles for classifying non-pathogenic Neisseria species.

    Main Methods:

    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to resolve and analyze cell envelope proteins.

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  • Examination of protein localization in outer membrane and periplasmic fractions.
  • Analysis of protein profiles under different growth conditions and in mutant strains.
  • Comparative analysis of protein patterns across eight non-pathogenic Neisseria species.

    • Main Results:

    • Approximately 20 cell envelope proteins were identified in N. sicca, primarily localized to the outer membrane.
    • Outer membrane protein composition was sensitive to sample solubilization temperature and growth medium.
    • Periplasmic proteins were found to associate with free endotoxin under phosphate limitation.
    • Mutations affecting antibiotic permeability correlated with changes in cell envelope protein composition.
    • SDS-PAGE protein patterns provided a characteristic fingerprint for each of the eight Neisseria species studied.

    Conclusions:

    • Cell envelope protein analysis via SDS-PAGE is a valuable tool for characterizing Neisseria species.
    • The protein profiles of the outer membrane and associated fractions reflect the genetic makeup and physiological state of the bacteria.
    • This proteomic approach aligns with traditional classification methods, offering a complementary strategy for bacterial taxonomy.