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Updated: Jul 28, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

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Published on: November 15, 2013

Analysis of receptor/ligand interactions using whole-molecule randomly-mutated ligand libraries

S A Cain1, C F Ratcliffe, D M Williams

  • 1Krebs Institute of Biomolecular Science, Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2UH, Sheffield, UK.

Journal of Immunological Methods
|October 24, 2000
PubMed
Summary

Researchers developed a new method to analyze protein ligands using whole molecule mutagenesis and phage display. This technique enhances protein-receptor binding affinity by identifying critical mutations.

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