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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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[Comparative immunochemical characteristics of polyclonal and monoclonal antibodies to aflatoxin B1]

M A Burkin1, I V Iakovleva, V V Sviridov

  • 1Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia.

Prikladnaia Biokhimiia I Mikrobiologiia
|October 24, 2000
PubMed

Four hybrid clones (MM-(AB1)-1, MM-(AB1)-2, MM-(AB1)-3, and MM-(AB1)-4) were obtained by hybridoma technology with immunization of BALB/c mice with a BSA conjugate of aflatoxin B1 carboxymethyloxime derivative. Antibodies produced by these clones varied in the ability to recognize the aflatoxin B1 analogues. The sensitivity of enzyme immunoassay based on all monoclonal antibodies was higher compared to analysis based on polyclonal rabbit antibodies (0.1 and 0.4 ng/ml, respectively).

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