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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

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Invertase fusion proteins for analysis of protein trafficking in yeast

T Darsow1, G Odorizzi, S D Emr

  • 1Department of Biology, University of California, San Diego, La Jolla 92093-0668, USA.

Methods in Enzymology
|October 25, 2000
PubMed
Abstract

No abstract available in PubMed .

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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