Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intestinal amoebiasis: delayed-type hypersensitivity response in mice.

P K Ghosh1, S Gupta, L Ortiz-Ortiz

  • 1Department of Immunology, Universidad Nacional Autonoma de Mexico, Mexico D.F.

Journal of Health, Population, and Nutrition
|November 1, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The global invasion risk of rice yellow stem borer Scirpophaga incertulas Walker (Lepidoptera:Crambidae) under current and future climate scenarios.

PloS one·2025
Same author

Impact of ultrasonic assisted triangular lattice like arranged dispersion of nanoparticles on physical and mechanical properties of epoxy-TiO<sub>2</sub> nanocomposites.

Ultrasonics sonochemistry·2018
Same author

MWCNT/TiO<sub>2</sub> hybrid nano filler toward high-performance epoxy composite.

Ultrasonics sonochemistry·2017
Same author

Microfilariæ in the Sputum.

The Indian medical gazette·2017
Same author

Spontaneous Pneumothorax with a Pleuro-Peritoneal Fistula.

The Indian medical gazette·2017
Same author

Tuberculosis of the Breast.

The Indian medical gazette·2017

Mice infected with Entamoeba histolytica showed a delayed-type hypersensitive (DTH) response, particularly evident 25 days post-infection. This cellular immune response was specific to the amoeba antigen, indicating no general immune suppression.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Parasitology

Background:

  • Entamoeba histolytica is an intestinal parasite causing amoebiasis.
  • Understanding the host's immune response, specifically delayed-type hypersensitivity (DTH), is crucial for evaluating infection dynamics.

Purpose of the Study:

  • To investigate the development and kinetics of the DTH response in C3H/HeJ mice infected with Entamoeba histolytica.
  • To determine if the observed DTH response is antigen-specific.

Main Methods:

  • C3H/HeJ mice were intestinally infected with Entamoeba histolytica.
  • Infected and control mice were challenged with amoebic antigen at various time points post-infection (days 5-60).
  • Footpad swelling was measured 24 hours post-challenge to assess DTH response.

Related Experiment Videos

Main Results:

  • Infected mice demonstrated a DTH response on days 5, 15, 20, 25, 40, and 60 post-infection.
  • A clear DTH response emerged later in the infection course, around 25 days.
  • No alteration in DTH response to a heterologous antigen (sheep red blood cells) was observed, suggesting antigen-specific immunity.

Conclusions:

  • Intestinal Entamoeba histolytica infection induces a delayed-type hypersensitive immune response in mice.
  • The DTH response is antigen-specific, indicating that the parasite does not induce broad cellular anergy.
  • The findings contribute to understanding the host-parasite immune interactions in amoebiasis.