Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium channel current in rat dental pulp cells.

R M Davidson1, L Guo

  • 1Division of Basic Sciences, 345 East 24th Street, New York University College of Dentistry, New York, NY 10010-4087, USA.

The Journal of Membrane Biology
|November 4, 2000
PubMed
Summary

Researchers identified two types of calcium currents (T-type and L/N-type) in rat dental pulp cells. These voltage-gated calcium channels may play a role in forming reparative dentin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Seroprevalence and risk factors for Toxoplasma gondii in sheep and goats in Jinzhou, Northeastern China.

Tropical biomedicine·2015
Same author

Relationship between epicardial adipose tissue volume and atrial fibrillation : A systematic review and meta-analysis.

Herz·2015
Same author

Cross Sections for the Exclusive Photon Electroproduction on the Proton and Generalized Parton Distributions.

Physical review letters·2015
Same author

Common variants in the Na(+)-coupled bicarbonate transporter genes and salt sensitivity of blood pressure: the GenSalt study.

Journal of human hypertension·2015
Same author

Glucocorticoids impair bone formation of bone marrow stromal stem cells by reciprocally regulating microRNA-34a-5p.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
Same author

Measurements of Elliptic and Triangular Flow in High-Multiplicity 3He+Au Collisions at √(s(NN))=200 GeV.

Physical review letters·2015

Area of Science:

  • Biophysics
  • Cell Biology
  • Dental Research

Background:

  • Dental pulp cells possess voltage-gated calcium channels.
  • Understanding these channels is crucial for dental pulp biology and regeneration.

Purpose of the Study:

  • To characterize voltage-gated calcium currents in early-passage rat dental pulp cells.
  • To identify the specific types of calcium channels present and their potential roles.

Main Methods:

  • Whole-cell patch-clamp electrophysiology was used to record currents.
  • Pharmacological agents (lanthanum, Bay-K, nifedipine, omega-Conotoxin GVIA) were employed to differentiate channel types.
  • Kinetic analysis distinguished between fast and slow inactivating currents.

Main Results:

  • Two distinct calcium currents, I(f) and I(s), were identified.
  • I(f) exhibited fast inactivation and was blocked by lanthanum, suggesting T-type calcium channels.
  • I(s) showed slow inactivation, was sensitive to L-type channel modulators, and blocked by omega-Conotoxin GVIA, indicating L- and N-type calcium channels.

Conclusions:

  • Rat dental pulp cells express T-type, L-type, and N-type voltage-gated calcium channels.
  • These channels are likely involved in the mineral-inductive properties of dental pulp cells and reparative dentin formation.

Related Experiment Videos