Synthesis of carbonic anhydrase in rabbit and chicken reticulocyte lysates

The synthesis of carbonic anhydrase, the second most abundant soluble protein of red blood cells, is shown to occur in rabbit and chicken reticulocyte lysates. This translation product was identified by chloroform/ethanol extraction, polyacrylamide gel electrophoresis in sodium dodecylsulphate and peptide mapping. In rabbit retic-locyte lysates, predominantly one of the two red cell isozymes, carbonic anhydrase I, is synthesised. The proportion of carbonic anhydrase synthesis (0.2-0.8% of total protein synthesis) in vitro is comparable to that (0.2-1.0%) in vivo for both rabbit and chicken reticulocytes.