Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Efficient DNA electrotransfer into tumors.

M Bettan1, M A Ivanov, L M Mir

  • 1UMR 70001 CNRS-ENSCP/Aventis, Vitry-sur-Seine, France.

Bioelectrochemistry (Amsterdam, Netherlands)
|November 4, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The role of perforator flaps in the management of pelvic-trochanteric pressure ulcers. Literature review and proposal of a treatment algorithm].

Annales de chirurgie plastique et esthetique·2025
Same author

Hair reconstruction of the burned chin region using a pre-expanded free temporo-parietal skin flap. About a case.

Annales de chirurgie plastique et esthetique·2024
Same author

[Sociological aspects of transgenrism].

Annales de chirurgie plastique et esthetique·2023
Same author

[Burns caused by nitrous oxide cylinder misuse].

Annales de chirurgie plastique et esthetique·2023
Same author

Size-switchable polymer-based nanomedicines in the advanced therapy of rheumatoid arthritis.

Journal of controlled release : official journal of the Controlled Release Society·2022
Same author

Vascularization of the superficial circumflex iliac perforator flap (SCIP flap): an anatomical study.

Surgical and radiologic anatomy : SRA·2020
Same journal

Energy-equivalent cyclic pulsed electric fields enable reversible membrane permeabilization and sustainable protein release from microalgae.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same journal

Electrochemical monitoring of electroactive compounds secreted by Escherichia coli during the aerobic-to-anaerobic transition.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same journal

Bioelectrochemical Sensing Dynamics of SARS-CoV-2 Biomarkers.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same journal

Meta-analysis and interpretable machine learning model of organic removal and power generation in photosynthetic microbial fuel cells.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same journal

Surfactant-doped PEDOT films as dual-function bioelectronic coatings with enhanced charge storage capacity and antibiofilm activity.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same journal

Design and application of a FA-based molecularly imprinted sensor for screening anti-Fusariumoxysporum substances from Lanzhou lily endophytes.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
See all related articles

Electrically mediated gene transfer significantly enhances DNA delivery to tumor cells, boosting therapeutic gene expression. This electrotransfer method shows great promise for improving cancer gene therapy effectiveness.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Oncology

Background:

  • Gene therapy for cancer involves delivering therapeutic genes to tumor cells.
  • Intratumoral plasmid DNA delivery often results in low gene expression levels.
  • Electrically mediated gene transfer has emerged as a method to enhance gene expression.

Purpose of the Study:

  • To evaluate the efficacy of long-duration electric pulses for gene transfer in various tumor models.
  • To quantify the increase in gene expression achieved through electrotransfer compared to conventional methods.
  • To confirm the potential of electrotransfer as a tool for cancer gene therapy.

Main Methods:

  • Intratumoral injection of plasmid DNA (luciferase or beta-Gal reporter genes) into murine and human tumor models.

Related Experiment Videos

  • Application of eight electric pulses (20-ms duration, 1-Hz frequency) using parallel electrodes.
  • Optimization of voltage-to-distance ratio for maximal gene transfer (400–600 V/cm).
  • Assessment of gene expression via luciferase activity and histological analysis of beta-Gal positive cells.

    • Main Results:

    • Electrotransfer increased gene expression 10- to 1200-fold compared to naked DNA injection.
    • Luciferase expression ranged from 0.6 to 300 ng per tumor two days post-electrotransfer.
    • Histological analysis showed a substantial increase in beta-Gal positive cells in H1299 tumors after electrotransfer.

    Conclusions:

    • Long-duration electric pulses effectively enhance DNA transfer and gene expression in tumor cells.
    • Electrotransfer significantly improves gene delivery efficiency for potential cancer gene therapy applications.
    • This technique offers a promising strategy to overcome limitations of current gene delivery methods in oncology.