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Monoclonal antibody binding to a surface-exposed epitope on Cowdria ruminantium that is conserved among eight strains
S Shompole1, F R Rurangirwa, A Wambugu
1Biotechnology and Immunology Laboratory, Kenya Agricultural Research Institute, Kabete, Kenya. shompole@vetmed.wsu.edu
Clinical and Diagnostic Laboratory Immunology
|November 4, 2000
Abstract:
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (EB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact EB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 bound an antigen of approximately 43 kDa in immunoblots of eight geographically distinct strains. The MAb did not react with Ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may be useful in diagnostic assays and vaccine development.