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Progesterone side-chain cleavage by Bacillus sphaericus.

L Wadhwa1, K E Smith

  • 1Molecular and Cellular Biology, Division of Biomedical Sciences, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK.

FEMS Microbiology Letters
|November 7, 2000
PubMed
Summary
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Bacillus sphaericus cleaved progesterone into androstenedione and 1-dehydroandrostenedione. High glucose levels in the culture medium inhibited this steroid conversion process.

Area of Science:

  • Microbiology
  • Biochemistry
  • Steroid Metabolism

Background:

  • Progesterone is a key steroid hormone.
  • Microbial biotransformation offers novel pathways for steroid modification.
  • Understanding steroid metabolism is crucial for pharmaceutical development.

Purpose of the Study:

  • To investigate the microbial degradation of progesterone by Bacillus sphaericus.
  • To identify the resulting C-19 keto androstene steroid metabolites.
  • To determine the effect of glucose concentration on progesterone biotransformation.

Main Methods:

  • Incubation of progesterone with Bacillus sphaericus.
  • Analysis of culture medium for steroid metabolites using analytical techniques.
  • Varying glucose concentrations in the culture medium to assess inhibitory effects.

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Main Results:

  • Bacillus sphaericus successfully cleaved the progesterone side-chain.
  • The primary metabolites identified were androstenedione and 1-dehydroandrostenedione.
  • Elevated glucose concentrations significantly inhibited the conversion of progesterone to these steroids.

Conclusions:

  • Bacillus sphaericus is capable of producing C-19 keto androstene steroids from progesterone.
  • Glucose acts as an inhibitor in the microbial metabolism of progesterone.
  • These findings contribute to the understanding of steroid biotransformation pathways.