Related Experiment Video
Updated: Jun 25, 2026

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
Published on: October 6, 2022
The Type III Secretion System plasmid pPHDPT3 of Photobacterium damselae subsp. piscicida is stable in Australian
Oleksandra Rudenko1, Laura Baseggio1, Andrew C Barnes1,2
1School of the Environment, The University of Queensland, Brisbane QLD 4072, Australia.
Abstract:
Photobacterium damselae subsp. piscicida (Pdp) is a host-adapted primary pathogen impacting finfish aquaculture worldwide, whose virulence evolution is driven by the mobilome. The pPHDPT3 plasmid encoding a type III secretion system is critical for Pdp virulence but unstable in vitro in European and Japanese isolates. Here we show that a stable ancestral variant is conserved in Australian isolates. The elusive pPHDPT3 variant has undergone gene loss and accumulated a ∼5.5 kb quadruple direct repeat, which could explain plasmid loss via the classic dimer catastrophe scenario. In addition, we hypothesize that a 189-aa serine recombinase encoded within this repeat, and also on pPHDP10 plasmid, may act as a plasmid resolvase, with its frequent loss further exacerbating the dimer catastrophe.
More Related Videos
11:35Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
Related Concept Videos
Plasmids
DNA Bacteriophages
Gram-negative Bacterial Protein Secretion Systems
Cytoskeletal Proteins in Bacteria
Bacterial Phylum Tenericutes
Bacterial Phylum Planctomycetes