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Related Experiment Videos

S100-annexin complexes: some insights from structural studies.

A Lewit-Bentley1, S Réty, J Sopkova-de Oliveira Santos

  • 1LURE (CNRS, CEA, MENRT), Centre Universitaire Paris-Sud, Orsay, 91898, France. anita@lure.u-psud.fr

Cell Biology International
|November 9, 2000
PubMed
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This study reveals the crystal structures of annexin N-terminus complexes, including annexin II/p11 and annexin I/S100C. These findings provide a model for the annexin II/p11 complex, aiding understanding of membrane fusion.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Annexins are a family of calcium-binding proteins.
  • Several annexins bind S100 calcium-binding proteins.
  • Annexin II/p11 complex is implicated in membrane fusion.

Purpose of the Study:

  • To determine the crystal structures of p11 with annexin II N-terminus and S100C with annexin I N-terminus.
  • To propose a computer-generated model for the entire annexin II/p11 complex.

Main Methods:

  • X-ray crystallography to solve complex structures.
  • Electron microscopy of liposome junctions.
  • Computer modeling for complex structure generation.

Main Results:

Related Experiment Videos

  • Crystal structures of p11/annexin II N-terminus and S100C/annexin I N-terminus were solved.
  • A computer model of the entire annexin II/p11 complex was generated.
  • Structural data was correlated with electron microscopy observations of liposome fusion.

Conclusions:

  • The solved crystal structures provide key insights into annexin-S100 protein interactions.
  • The proposed model advances understanding of the annexin II/p11 complex's role in membrane fusion.
  • This research contributes to the structural understanding of calcium-binding protein interactions.