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Published on: October 13, 2010
Quantification of glycoalkaloids in tomato plants by time-resolved fluorescence using a europium chelator entrapped
M A Bacigalupo1, A Ius, R Longhi
1Istituto di Biocatalisi e Riconoscimento Molecolare, CNR, Milan, Italy. bacigalupo@ico.mi.cnr.it
Abstract:
In the control of tomato transgenic cultivars it is important to determine the glycoalkaloid content, which requires the screening of many samples. We have developed a simple method for the extraction and determination of glycoalkaloids in the leaf and fruit extracts of tomatoes using a europium chelator entrapped in phosphatidylcholine-cholesterol containing liposomes. The concentration values were quantified from liposome lysis effected by glycoalkaloid action. The fluorescent signal is linear between 1 and 10 micrograms of tomatine, which makes the method useful for the analysis of tomato samples.
