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Effect of hyperoxia on vascular endothelial growth factor levels in a wound model
A Y Sheikh1, J J Gibson, M D Rollins
1Department of Surgery, University of California, San Francisco, 513 Parnassus Ave, Box 0522, HSW 1652, San Francisco, CA 94143-0522, USA.
Hypothesis:
Hyperbaric oxygen (HBO) therapy increases vascular endothelial growth factor (VEGF) levels in wounds.
Design:
Wounds were monitored for oxygen delivery during HBO treatment, and wound fluids were analyzed for VEGF and lactate on days 2, 5, and 10 following wounding.
Setting:
Experimental animal model.
Interventions:
Rats were randomized to HBO therapy and control groups. The HBO therapy was administered for 90 minutes, twice daily with 100% oxygen at 2.1 atmospheres absolute. Treatment was administered for 7 days following wounding.
Main Outcome Measures:
Vascular endothelial growth factor, PO(2), and lactate levels in wound fluid were measured on days 2, 5, and 10.
Results:
Wound oxygen rises with HBO from nearly 0 mm Hg to as high as 600 mm Hg. The peak level occurs at the end of the 90-minute treatment, and hyperoxia of lessening degree persists for approximately 1 hour. The VEGF levels significantly increase with HBO by approximately 40% 5 days following wounding and decrease to control levels 3 days after exposures are stopped. Wound lactate levels remain unchanged with HBO treatment (range, 2.0-10.5 mmol/L).
Conclusions:
Increased VEGF production seems to explain in part the angiogenic action of HBO. This supports other data that hypoxia is not necessarily a requirement for wound VEGF production.