Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput expression of fusion proteins.

M Nasoff1, M Bergseid, J P Hoeffler

  • 1Genomics Institute of the Novartis Research Foundation, San Diego, California 92121-1125, USA.

Methods in Enzymology
|November 15, 2000
PubMed
Summary

Epitope tagging facilitates the rapid identification and purification of functional recombinant plasmids for protein research. This method accelerates downstream applications like protein purification and immunolocalization studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selection of transfected mammalian cells.

Current protocols in neuroscience·2008
Same author

Small molecule-based chemical affinity system for the purification of proteins.

BioTechniques·2000
Same author

In vivo selection of single-chain antibodies using a yeast two-hybrid system.

Journal of immunological methods·2000
Same author

The transcriptional response of yeast to saline stress.

The Journal of biological chemistry·2000
Same author

Identification of the immunoreactive peptide sequence for AgSK1, an adenocarcinoma-restricted antigen.

Tissue antigens·2000
Same author

Co-expression of tumor antigens and their modulation by pleiotrophic modifiers enhance targeting of human monoclonal antibodies to pancreatic carcinoma.

Human antibodies·1999

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Protein Expression

Background:

  • Fusion protein expression accelerates research.
  • Epitope tags enable efficient identification of recombinant plasmids via Western blotting.

Purpose of the Study:

  • To present protocols for utilizing epitope tags in molecular biology research.
  • To demonstrate the utility of epitope tags in identifying functional plasmids.

Main Methods:

  • Expression of open reading frames (ORFs) as fusion proteins.
  • Utilizing Western blotting for identification of recombinant plasmids.
  • Application of protocols to various expression vectors.

Main Results:

  • Epitope tags streamline the identification of useful recombinant plasmids.

Related Experiment Videos

  • Protocols are adaptable for high-throughput assays and downstream applications.

    • Conclusions:

    • Epitope tagging is a versatile tool for protein research.
    • The presented protocols are accessible and enhance protein expression studies.