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IGF-II promotes mesoderm formation.

O G Morali1, A Jouneau, K J McLaughlin

  • 1Subcellular Structure and Cellular Dynamics, UMR 144 CNRS-Institut Curie, 26 rue d'Ulm, Paris Cedex 05, 75248, France.

Developmental Biology
|November 15, 2000
PubMed
Summary
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Insulin-like growth factor II (IGF-II) is crucial for mesoderm development in early mouse embryos. This study shows IGF-II signaling through its receptor IGF1R promotes mesoderm cell formation and differentiation.

Area of Science:

  • Developmental Biology
  • Genetics
  • Cell Biology

Background:

  • Insulin-like growth factor II (IGF-II) is present in early mouse embryonic mesoderm, but its function is unclear.
  • Igf2 exhibits genomic imprinting, being paternally expressed in most mouse embryonic tissues.

Purpose of the Study:

  • To investigate the role of IGF-II in mesoderm development during early embryogenesis.
  • To determine if IGF-II levels influence mesoderm differentiation in vitro and in vivo.

Main Methods:

  • Utilized various murine embryonic stem (ES) cell lines (androgenetic, biparental, parthenogenetic, Igf2-/-) with differing endogenous IGF-II levels.
  • Assessed mesoderm marker expression and muscle structure formation during teratoma formation and in vitro differentiation.
  • Administered exogenous IGF-II to ES cell cultures.

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Main Results:

  • Endogenous IGF-II levels correlated positively with mesoderm marker expression and muscle formation.
  • Absence of Igf2 in androgenetic Igf2-/- ES cells severely impaired mesoderm development.
  • Exogenous IGF-II addition specifically enhanced mesoderm marker expression in vitro.

Conclusions:

  • IGF-II is essential for proper mesoderm development in early mouse embryos.
  • Androgenetic ES cells' preferential mesoderm development depends on Igf2 activity.
  • A model is proposed where IGF-II binding to IGF1R on mesoderm precursors promotes mesoderm cell formation.